New York, USA – June 23, 2021 – Microbiosci, the microbiology platform of Creative Biogene, is dedicated to developing unique technologies to facilitate the investigation of microbial researches. With a wide range of high quality products and services, Microbiosci also delivers medicine microbiology solutions to clinical, pharmaceutical and veterinary. Recently, Microbiosci released optimized real-time PCR kits that can be applied for RNA detection, molecular cloning, and gene sequencing.
Microbiosci platform designs products that can be used in real-time (quantitative) PCR programs, including master mix, DNA polymerase, reverse transcriptase, size standards, kits and reagents. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that reverse transcribes RNA into DNA and then uses PCR to amplify the DNA.
In RT-PCR, reverse transcriptase converts the RNA template into its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification. A typical RT-PCR kit includes the main components required for testing:
Primer-a combination of four bases of deoxynucleotide triphosphate (dNTP) of adenine (dATP), cytosine (dCTP), guanine (dGTP) and thymine (dTTP)
Reverse transcriptase reagent
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray verification, pathogen detection, genetic testing and other disease research. A combination of RT-PCR and qPCR technology (sometimes labeled RT-qPCR) is commonly used in research and clinical laboratories to analyze gene expression and quantify viral RNA. Because this method uses fluorescence to track amplification reactions, these kits may also include Fluorescent primers or labeled fluorescent probes and Fluorescent dyes. One-step RT-PCR is most commonly used to process multiple samples at once, with each sample processing one or several targets. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.
When performing real-time PCR, the success of the experiment depends on many key factors, including enhanced specificity, sensitivity, speed, ease of use, and process safety. In addition, customers are often under pressure to use internal controls to preserve precious samples, increase throughput, and obtain reliable results.
“Microbiosci’s real-time PCR kit can help you overcome these challenges and demonstrate high specificity through a novel hot start mechanism, allowing you to detect as few as one copy of the target sequence-in addition, HRM solutions are also available. Thanks to the built-in visual indicator in our qPCR kit, you can ensure precise reaction settings.” said Marcia Brady, she also added, “Based on the built-in visual indicator in our qPCR kit, customers can get precise reaction settings.”
Microbiosci, as the division of Creative Biogene, is always dedicated to satisfying the needs of clients covering more than 50 countries and districts. As a leading custom service provider in delivering medicine microbiology solutions, Microbiosci has become a well-recognized industry leader with years of experience and professional scientists.